This antibody specifically recognizes the C-terminal region of human GPR39, detecting the non-phosphorylated isoform. It is validated for Western blot analysis, enables immunoprecipitation from tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPR39. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of GPR39 Receptor (GPR39) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPR39 were immunoblotted with the phosphorylation-independent anti-GPR39 antibody (A334465) at a 1:1000 dilution.
Immunohistochemical detection of GPR39 Receptor (GPR39) in kidney. Kidney sections were dewaxed, microwaved in citric acid, and stained with anti-GPR39 antibody (A334465) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Localization of GPR39 Receptor (GPR39) in pancreas by immunohistochemistry. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR39 antibody (A334465) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Immunohistochemical detection of GPR39 Receptor (GPR39) in small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and stained with anti-GPR39 antibody (A334465) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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