This antibody binds specifically to the C-terminal domain of human GPR91, detecting the non-phosphorylated isoform. It is suitable for Western blot analysis, facilitates immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPR91. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of SUCNR1/GPR91 Receptor (GPR91) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPR91 were probed with the phosphorylation-independent anti-SUCNR1/GPR91 antibody (A334556) at a 1:1000 dilution.
Verification of SUCNR1/GPR91 Receptor (GPR91) antibody specificity in pancreas by immunohistochemistry. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated without (left panel) or with (right panel) the peptide used to generate anti-GPR91 antibody (A334556). Sections were stained with anti-SUCNR1/GPR91 antibody (A334556) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution, then developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. GPR91 receptors were detected at the plasma membrane only in sections without peptide incubation.
Immunohistochemical detection of SUCNR1/GPR91 Receptor (GPR91) in neuroendocrine cells. Sections were dewaxed, microwaved in citric acid, and stained with anti-GPR91 antibody (A334556) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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