Mouse monoclonal [GB10] antibody to Granzyme B (Biotin).
Specificity
This antibody recognises the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.
Applications
ELISA
Dilutions
ELISA: 1 µg/ml
Reactivity
Human, Chimpanzee, Monkey, Rhesus Monkey
Immunogen
Purified human Granzyme B.
Host
Mouse
Clonality
Monoclonal
Clone ID
GB10
Isotype
IgG1
Conjugate
Biotin
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human granzyme B antibody, clone GB10 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.
Anti-Granzyme B Antibody [GB10] (Biotin) (A280503)
Sandwich ELISA analysis of human granzyme B using Anti-Granzyme B Antibody [GB11] (A280504) as a capture reagent and Anti-Granzyme B Antibody [GB10] (Biotin) (A280503) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Human plasma samples are diluted i:2 (green) and 1:4 (blue)
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