This antibody recognizes GRB2, a highly conserved approximately 26 kDa adaptor protein. The immunogen has 100% identity between human, bovine, rat, and mouse.
Applications
WB
Dilutions
WB: 1-2 µg/ml; Reducing conditions.
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminus, amino acids 198-217, of human GRB2.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
1 mg/ml
Predicted MW
26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Adapter protein GRB2, ASH, Growth factor receptor-bound protein 2, Protein Ash, SH2/SH3 adapter GRB2
Western bloting analysis of human GRB2 using rabbit polyclonal antibody PAb (554) on lysates of MOLT-4 and RAMOS cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml using Anti-GRB2 Antibody (A86507) followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for GRB2 at approximately 24 kDa.
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