Anti-Grp75/MOT Antibody (A12650)

Name: Anti-Grp75/MOT Antibody
Brand: Antibodies.com
SKU: A12650

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This product is discontinued

Anti-Grp75/MOT Antibody (A12650) has been discontinued and is no longer available.

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Specifications

Name

Anti-Grp75/MOT Antibody

Description

Rabbit polyclonal antibody to Grp75/MOT.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human Grp75/MOT/Grp75/MOT/HSPA9 (NP_004125.3).

Sequence

IGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Grp75/MOT Antibody (A12650)

Western blot analysis of extracts of various cell lines, using Anti-Grp75/MOT Antibody (A12650) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Immunohistochemistry - Anti-Grp75/MOT Antibody (A12650)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Grp75/MOT Antibody (A12650) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Grp75/MOT Antibody (A12650)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Grp75/MOT Antibody (A12650) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Grp75/MOT Antibody (A12650)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-Grp75/MOT Antibody (A12650) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.