GST3/GST pi expression in CaCo-2 (A), K562 (B), HeLa (C), HEK293 (D), Jurkat (E), NIH3T3 (F), KNRK (G), and MDCK (H) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-GST3/GST pi Antibody (A84101) at 0.3µg/ml (A-B) or 0.03µg/ml (C-H) and detected by chemiluminescence.
GST3/GST pi expression in HeLa (A), HEK293 (B), and Jurkat (C) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-GST3/GST pi Antibody (A84101) at 0.1µg/ml (A-B) or 0.03µg/ml (C) and detected by chemiluminescence.
GST3/GST pi expression in Human Thymus (A), Lung (B), Kidney (C), lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GST3/GST pi Antibody (A84101) at 0.3µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-GST3/GST pi Antibody (A84101)
GST3/GST pi expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-GST3/GST pi Antibody (A84101) at 5µg/ml and revealed with alkaline phosphatase (AP).
Immunofluorescence - Anti-GST3/GST pi Antibody (A84101)
GST3/GST pi expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GST3/GST pi Antibody (A84101) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Immunofluorescence - Anti-GST3/GST pi Antibody (A84101)
GST3/GST pi expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GST3/GST pi Antibody (A84101) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Flow Cytometry - Anti-GST3/GST pi Antibody (A84101)
GST3/GST pi expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-GST3/GST pi Antibody (A84101) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
