This antibody reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Applications
WB
Dilutions
WB: 1-2 µg/ml; Reducing conditions.
Reactivity
Human
Immunogen
Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.
Sequence
DIHQYREQIKRVKDSDDC
Host
Mouse
Clonality
Monoclonal
Clone ID
H-RAS-03
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
21 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blotting analysis of human H-Ras using Anti-H-RAS Antibody [H-RAS-03] (A86021) on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.
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