HA-Tag Mouse Monoclonal antibody HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with HA-tag protein C: IP (HEK 293 cell without transfected + anti- HA mAb 4℃ overnight) D: IP (PBS + anti- HA mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- HA mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain
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