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Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com
This product is discontinued

Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-HA Tag Antibody [AMC0512] (HRP)
Description
Mouse monoclonal [AMC0512] antibody to HA Tag.
Applications
WB
Dilutions
WB: 1:500-1:5,000
Reactivity
Species Independent
Immunogen
A synthetic peptide corresponding to HA tag.
Sequence
YPYDVPDYA
Host
Mouse
Clonality
Monoclonal
Clone ID
AMC0512
Isotype
IgG1
Light Chains
kappa
Conjugate

HRP

Purification
Affinity purification.
Molecular Weight
53 kDa (CD95) / 65 kDa (CD2) / 53 kDa (SCRN1) 42 kDa (WDR77) / 45 kDa (NR3C2)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)

Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein, using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein, 293F transfected with CD95-N Protein using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 90s.