Western blot analysis of extracts of K-562 cells, using Anti-HADHA Antibody (A14688) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using Anti-HADHA Antibody (A14688) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Confocal immunofluorescence analysis of U-2 OS cells using Anti-HADHA Antibody (A14688) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-HADHA Antibody (A14688). This Western blot was performed on the immunoprecipitate using Anti-HADHA Antibody (A14688) at a dilution of 1:1000.
