Hamartin expression in Human Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Hamartin Antibody (A83253) at 0.1µg/ml and detected by chemiluminescence.
Hamartin expression in Human Uterus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Hamartin Antibody (A83253) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
Hamartin expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Hamartin Antibody (A83253) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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