Anti-HDAC2 (phospho Ser394) Antibody (A16510)

Western blot analysis of HepG2, using Anti-HDAC2 (phospho Ser394) Antibody (A16510) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-HDAC2 (phospho Ser394) Antibody (A16510) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-HDAC2 (phospho Ser394) Antibody (A16510) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of methanol-fixed HeLa cells showing nuclear staining using Anti-HDAC2 (phospho Ser394) Antibody (A16510).