ELISA analysis of human HDAC6 using Anti-HDAC6 Antibody [159] (A242877). MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of Anti-HDAC6 Antibody [159] (A242877), followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) using Anti-HDAC6 Antibody [159] (A242877) , followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).
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