This antibody recognizes the D2 domain of human Histone Deacetylase 6 (intracellular antigen), amino acids 471-481. Cross-reactivity with other species was not tested.
Applications
WB, ICC, ELISA
Dilutions
WB: 1-5 µg/ml, IHC: 5-10 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse or Danio.
Immunogen
Human HDAC6.
Host
Mouse
Clonality
Monoclonal
Clone ID
3D2
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) using Anti-HDAC6 Antibody [3D2] (A285871) , followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).
ELISA analysis of human HDAC6 using Anti-HDAC6 Antibody [3D2] (A285871). MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of Anti-HDAC6 Antibody [3D2] (A285871), followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
Western blotting analysis of human HDAC6 using Anti-HDAC6 Antibody [3D2] (A285871)in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.
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