Rabbit polyclonal antibody to Hemoglobin subunit alpha.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Hemoglobin subunit alpha (Hemoglobin subunit alpha (HBA1)) (NP_000508.1).
Western Blot - Anti-Hemoglobin subunit alpha Antibody (A10113)
Western blot analysis of extracts of various cell lines, using Anti-Hemoglobin subunit alpha Antibody (A10113) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 30s.
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