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Anti-HIF-3 alpha Antibody (A268189)

Validation Data - Anti-HIF-3 alpha Antibody (A268189) Validation Data - Anti-HIF-3 alpha Antibody (A268189)
$565

Rabbit polyclonal antibody to HIF-3 alpha for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-HIF-3 alpha Antibody
Description
Rabbit polyclonal antibody to HIF-3 alpha.
Specificity
Anti-HIF-3 alpha Antibody detects endogenous levels of total HIF-3 alpha.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ELISA: 1:20,000-1:40,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human HIF-3 alpha, corresponding to a region within the internal amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification, using SulfoLink™ Coupling Resin.
Concentration
1 mg/ml
Molecular Weight
72 kDa
Predicted MW
72 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol, 150 mM Sodium Chloride, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Validation Data - Anti-HIF-3 alpha Antibody (A268189)Enlarge Image

Anti-HIF-3 alpha Antibody (A268189)

Western blot analysis of HIF-3 alpha using COLO205 whole cell lysates
Validation Data - Anti-HIF-3 alpha Antibody (A268189)Enlarge Image

Anti-HIF-3 alpha Antibody (A268189)

DF8557 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.