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Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821)

Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) - Antibodies.com Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit monoclonal [ARC0826] antibody to HIF1AN/FIH-1 for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-HIF1AN/FIH-1 Antibody [ARC0826]
Description
Rabbit monoclonal [ARC0826] antibody to HIF1AN/FIH-1.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HIF1AN/FIH1 (Q9NWT6).
Sequence
MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKF
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0826
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) - Antibodies.comEnlarge Image

Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821)

Western blot analysis of extracts from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) - Antibodies.comEnlarge Image

Western Blot - Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821)

Western blot analysis of extracts of U-87MG cells, using Anti-HIF1AN/FIH-1 Antibody [ARC0826] (A306821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
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