Anti-HIGD1A Antibody (A88280)

Name: Anti-HIGD1A Antibody
Brand: Antibodies.com
SKU: A88280
Price: 270.0 GBP
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to HIGD1A for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-HIGD1A Antibody

Description

Rabbit polyclonal antibody to HIGD1A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2).

Sequence

MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

HIG1, HIG1 domain family member 1A, mitochondrial, Hypoxia-inducible gene 1 protein, RCF1 homolog A, RCF1a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

Enlarge Image

Western Blot - Anti-HIGD1A Antibody (A88280)

Western blot analysis of extracts of various cell lines, using Anti-HIGD1A Antibody (A88280) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Immunohistochemistry - Anti-HIGD1A Antibody (A88280)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HIGD1A Antibody (A88280) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Immunofluorescence - Anti-HIGD1A Antibody (A88280)

Immunofluorescence analysis of U-2 OS cells using Anti-HIGD1A Antibody (A88280) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-HIGD1A Antibody (A88280)? Please let us know so that we can list the citation on this page.