Anti-Histone H1.2 Antibody [ARC1836] (A307750)

Name: Anti-Histone H1.2 Antibody [ARC1836]
Brand: Antibodies.com
SKU: A307750
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1836] antibody to Histone H1.2 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Histone H1.2 Antibody [ARC1836]

Description

Rabbit monoclonal [ARC1836] antibody to Histone H1.2.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.2 (P16403).

Sequence

MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1836

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H1-2, H1F2, HIST1H1C, Histone H1c, Histone H1d, Histone H1s-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750)

Western blot analysis of extracts of various cell lines, using Anti-Histone H1.2 Antibody [ARC1836] (A307750) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750)

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Immunofluorescence - Anti-Histone H1.2 Antibody [ARC1836] (A307750)

Immunofluorescence analysis of U-2 OS cells using Anti-Histone H1.2 Antibody [ARC1836] (A307750) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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