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Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252] (A305307)

Western Blot - Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252] (A305307) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC0252] antibody to Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252]
Description
Rabbit monoclonal [ARC0252] antibody to Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T17 of human Histone H1.3H13 (P16402).
Sequence
AEKTP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0252
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
30 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252] (A305307) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252] (A305307)

Western blot analysis of extracts of various cell lines, using Anti-Histone H1.3 (phospho Thr17) + Histone H1.4 (phospho Thr17) Antibody [ARC0252] (A305307) at 1:1,000 dilution. Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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