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Recombinant Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243)

Multiplex Immunoassay - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243) - Antibodies.com Western Blot - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243) - Antibodies.com Immunocytochemistry - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121317) - Antibodies.com
Recombinant Antibody
$620
Product Datasheet

Recombinant rabbit monoclonal [RM216] antibody to Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) for WB, ELISA, Multiplex Protein Detection and ICC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216]
Description
Recombinant rabbit monoclonal [RM216] antibody to Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1).
Specificity
This antibody recognizes Histone H4 or Histone H2A phosphorylated at Serine 1.
Applications
WB, ELISA, Multiplex Protein Detection, ICC
Dilutions
WB: 0.5-2 µg/ml, ICC: 1-2 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.1-1 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other phosphorylated Histones.
Immunogen
Synthetic peptide corresponding to Histone H3 (N terminal).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM216
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Multiplex Immunoassay - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243) - Antibodies.comEnlarge Image

Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243)

Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] specifically reacts to both Histone H2A and Histone H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
Western Blot - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243)

Western blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] at 0.5µg/ml, showed both Histone H2A and Histone H4 phosphorylated Serine 1 in HeLa cells.
Immunocytochemistry - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121317) - Antibodies.comEnlarge Image

Immunocytochemistry - Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (A121243)

Immunocytochemistry of HeLa cells, using Anti-Histone H2A (phospho Ser1) + Histone H4 (phospho Ser1) Antibody [RM216] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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