Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Name: Anti-Histone H3 (tri methyl Lys36) Antibody
Brand: Antibodies.com
SKU: A16714
Price: 340.0 GBP
Availability: InStock

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This product is discontinued

Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) has been discontinued and is no longer available.

View all Histone H3 Antibodies.

Specifications

Name

Anti-Histone H3 (tri methyl Lys36) Antibody

Description

Rabbit polyclonal antibody to Histone H3 (tri methyl Lys36).

Applications

WB, IHC, ICC/IF, IP, ChIP, ChIP-seq

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:20-1:100, ChIP-seq: 1:20-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic trimethylated peptide around K36 of human histone H3 (NP_003520.1).

Sequence

GVKKP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (10)

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Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 180s.

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Dot Blot - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Dot blot analysis of a mixture of methylation peptides using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714).

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Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunofluorescence analysis of 293T cells using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714). DAPI was used to stain the cell nuclei (blue).

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Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa; cells, using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Enlarge Image

Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin immunoprecipitation (ChIP) analysis of extracts of Hale cells, using Anti-Histone H3 (tri methyl Lys36) Antibody (A16714) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Specifications

Scientific Validation DataValidation Data (10)

Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Dot Blot - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunohistochemistry - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Immunofluorescence - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody (A16714)

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