Anti-HLA B27 Antibody [HLA-ABC-m3] (FITC) (A280602)

Anti-HLA B27 Antibody [HLA-ABC-m3] (FITC)

Mouse monoclonal [HLA-ABC-m3] antibody to HLA B27 (FITC).

This antibody recognises the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).Flow cytometry - subjects expected fluorescence intensity:Heterozygous HLA-B27+ = Strong.Homozygous HLA-B27+ = Strong.Heterozygous HLA-B7+ = Faint.Non B27, Non B7 = Negative.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl of whole blood.

Human

Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A.

Mouse

Monoclonal

IgG2a

Protein A affinity chromatography of ascites.

50 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).Flow cytometry - subjects expected fluorescence intensity:Heterozygous HLA-B27+ = Strong.Homozygous HLA-B27+ = Strong.Heterozygous HLA-B7+ = Faint.Non B27, Non B7 = Negative.

This product is for research use only. It is not intended for diagnostic or therapeutic use.