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Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252018)

SDS-PAGE - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252018) - Antibodies.com Flow Cytometry - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252016) - Antibodies.com
$545
Product Datasheet

Mouse monoclonal [BRA-FB6] antibody to HLA DP for ELISA, Flow Cytometry and IF.

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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free
Description
Mouse monoclonal [BRA-FB6] antibody to HLA DP.
Specificity
This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Applications
ELISA, Flow Cytometry, IF
Dilutions
ELISA: 2-4 µg/ml, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml
Reactivity
Human
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
BRA-FB6
Isotype
IgG2b
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
33-35 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-HLA DP Antibody [BRA-FB6] (A248838).
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

SDS-PAGE - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252018) - Antibodies.comEnlarge Image

SDS-PAGE - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252018)

SDS-PAGE analysis of Anti-HLA DP Antibody [BRA-FB6] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Flow Cytometry - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252016) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-HLA DP Antibody [BRA-FB6] - BSA and Azide free (A252018)

Flow cytometric analysis of PFA fixed Raji cells using Anti-HLA DP Antibody [BRA-FB6] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
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