Recombinant rabbit monoclonal [HLA-DPB1/2862R] antibody to HLA DP.
Specificity
This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
SDS-PAGE analysis of Anti-HLA DP Antibody [HLA-DPB1/2862R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Flow cytometric analysis of Raji cells using Anti-HLA DP Antibody [HLA-DPB1/2862R] followed by Goat Anti-Rabbit IgG (CF® 488) (Blue). Isotype Control (Red).
Immunofluorescent analysis of Raji cells stained with Anti-HLA DP Antibody [HLA-DPB1/2862R] followed by Goat Anti-Rabbit IgG (CF® 488) (Green). Nuclear counterstain is RedDot (Red).
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