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Recombinant Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925)

Immunohistochemistry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.com SDS-PAGE - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.com Flow Cytometry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250924) - Antibodies.com Immunofluorescence - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.com
Recombinant Antibody
$545
Product Datasheet

Recombinant mouse monoclonal [rHLA-Pan/3475] antibody to HLA DP + DQ + DR for ELISA, Flow Cytometry, IF and IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475]
Description
Recombinant mouse monoclonal [rHLA-Pan/3475] antibody to HLA DP + DQ + DR.
Specificity
This antibody reacts with a common epitope of human major histocompatibility (MHC) class II antigens: HLA DP, HLA DQ, and HLA DR.
Applications
ELISA, Flow Cytometry, IF, IHC-P
Dilutions
ELISA: 1-5 µg/ml, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
rHLA-Pan/3475
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
33-35 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] - BSA and Azide free (A254105).
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Immunohistochemistry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil using Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475].
SDS-PAGE - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.comEnlarge Image

SDS-PAGE - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925)

SDS-PAGE analysis of Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Flow Cytometry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250924) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925)

Flow cytometric analysis of human Raji cells using Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
Immunofluorescence - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] (A250925)

Immunofluorescent analysis of Raji cells stained with Anti-HLA DP + DQ + DR Antibody [rHLA-Pan/3475] followed by Goat Anti-Mouse IgG (CF® 488) (Green). The nuclear counterstain is RedDot (Red).
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