Recombinant Anti-HLA DQ Antibody [HLA-DQA1/2866R] (A248846)

Anti-HLA DQ Antibody [HLA-DQA1/2866R]

Recombinant rabbit monoclonal [HLA-DQA1/2866R] antibody to HLA DQ.

This antibody recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Flow Cytometry, IF, IHC

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC: 1-2 µg/ml

Human, Porcine

Recombinant full-length human HLA DQ protein.

Rabbit

Monoclonal

HLA-DQA1/2866R

Protein A/G chromatography.

200 µg/ml

60 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-HLA DQ Antibody [HLA-DQA1/2866R] - BSA and Azide free (A252026).