Anti-HLA-DQA1 Antibody [ARC0564] (A305719)

Name: Anti-HLA-DQA1 Antibody [ARC0564]
Brand: Antibodies.com
SKU: A305719
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0564] antibody to HLA-DQA1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-HLA-DQA1 Antibody [ARC0564]

Description

Rabbit monoclonal [ARC0564] antibody to HLA-DQA1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 155-254 of human HLA-DQA1 (P01909).

Sequence

EGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSELTETVVCALGLSVGLVGIVVGTVFIIRGLRSVGASRHQGPL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0564

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DC-1 alpha chain, DC-alpha, HLA class II histocompatibility antigen, DQ alpha 1 chain, HLA-DCA, MHC class II DQA1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-HLA-DQA1 Antibody [ARC0564] (A305719)

Western blot analysis of extracts of various cell lines, using Anti-HLA-DQA1 Antibody [ARC0564] (A305719) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-HLA-DQA1 Antibody [ARC0564] (A305719)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-HLA-DQA1 Antibody [ARC0564] (A305719) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-HLA-DQA1 Antibody [ARC0564] (A305719)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-HLA-DQA1 Antibody [ARC0564] (A305719) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-HLA-DQA1 Antibody [ARC0564] (A305719)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-HLA-DQA1 Antibody [ARC0564] (A305719) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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