Anti-HLTF Antibody (A295358)

Name: Anti-HLTF Antibody
Brand: Antibodies.com
SKU: A295358
Price: 415.0 GBP
Availability: InStock

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$490

Rabbit polyclonal antibody to HLTF for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Specifications

Name

Anti-HLTF Antibody

Description

Rabbit polyclonal antibody to HLTF.

Applications

WB, IP

Dilutions

IP: 2-5 µg/1 mg lysate, WB: 1:3,000-1:10,000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide within amino acids 600-700 of human HLTF.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

DNA-binding protein/plasminogen activator inhibitor 1 regulator, Helicase-like transcription factor, HIP116, HIP116A, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, RNF80, SMARCA3, SNF2L3, Sucrose nonfermenting protein 2-like 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, ZBU1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-HLTF Antibody (A295358)

Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: HLTF Antibody (A295358) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Immunoprecipitation - Anti-HLTF Antibody (A295358)

Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: HLTF Antibody (A295358) was used for WB at 0.4 µg/ml and used for IP at 6 µg/mg lysate. HLTF was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure time of 10 seconds.

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Western Blot - Anti-HLTF Antibody (A295358)

Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: HLTF Antibody (A295358) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 3 minutes.

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