Maria Isabel Fernandez Pisonero, University of Salamanca
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Applications: Western Blot
Species: Mouse
Sample: Lung tissue and 4T1 breast cancer cells
Experimental Conditions: WB: Lysate BLYS buffer (50 mM Tris-HCl pH 7.5, 1 mM EGTA, 1 mM EDTA, 1% Triton-X100, 1 mM Na3VO4, 50 mM NaF, 5 mM pyrophosphate, 0.27 M sucrose, 1 mM PMSF, protease inhibitors); 40 µg protein; 10% acrylamide gel; nitrocellulose iBlot program 2, 6 min, 23 V; blocking 5% BSA 2 h RT; primary 1:1000 O/N; wash 3×10 min TBS-T; secondary Licor 926-32211 1:5000 1 h; detection LICOR fluorescence.
Dilution: WB: 1:1000
Results Summary: Lots of background with no defined band detected.
Comments: Antibody did not work under described conditions.
