Anti-HMGCS2 Antibody [ARC2390] (A305318)

Name: Anti-HMGCS2 Antibody [ARC2390]
Brand: Antibodies.com
SKU: A305318

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This product is discontinued

Anti-HMGCS2 Antibody [ARC2390] (A305318) has been discontinued and is no longer available.

View all HMGCS2 Antibodies.

Specifications

Name

Anti-HMGCS2 Antibody [ARC2390]

Description

Rabbit monoclonal [ARC2390] antibody to HMGCS2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 409-508 of human HMGCS2 (P54868).

Sequence

AFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2390

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3-hydroxy-3-methylglutaryl coenzyme A synthase, HMG-CoA synthase, Hydroxymethylglutaryl-CoA synthase, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-HMGCS2 Antibody [ARC2390] (A305318)

Western blot analysis of extracts of various cell lines, using Anti-HMGCS2 Antibody [ARC2390] (A305318) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-HMGCS2 Antibody [ARC2390] (A305318)

Western blot analysis of extracts of HepG2 cells, using Anti-HMGCS2 Antibody [ARC2390] (A305318) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-HMGCS2 Antibody [ARC2390] (A305318)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-HMGCS2 Antibody [ARC2390] (A305318) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.