Immunohistochemistry analysis of human prostate, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-HO-2 Antibody (A304991) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of mouse, rat brain showing detection of ~36 kDa HO-2 protein using Anti-HO-2 Antibody (A304991) at 1:1,000 for 2 hours at room temperature with shaking. Load: 15 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~36 kDa.
Immunohistochemistry analysis of human prostate, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-HO-2 Antibody (A304991) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
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