Anti-HP1 gamma/CBX3 (phospho Ser83) Antibody (A89585)

Western blot analysis of extracts of HeLa cells, using Anti-HP1 gamma/CBX3 (phospho Ser83) Antibody (A89585) at 1:1,000 dilution or HP1 gamma/CBX3 antibody (A2248). HeLa cells were treated by Paclitaxel (100 nM/mL) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

Western blot analysis of extracts of NIH/3T3 cells, using Anti-HP1 gamma/CBX3 (phospho Ser83) Antibody (A89585) at 1:1,000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 180s.