Synthetic peptide corresponding to the C-terminal end of human HSP73 (550-646 aa).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Molecular Weight
73 kDa
Purity
Whole antiserum.
Product Form
Liquid
Formulation
Supplied as an aliquot of antiserum.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein, Heat shock protein family A member 8, HSP73, HSPA10, HSPA8, LAP-1, Lipopolysaccharide-associated protein 1, LPS-associated protein 1
Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:50,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.
Western blot analysis of human Cell line lysates showing detection of HSC70 protein using Anti-HSC70 Antibody (A304779) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.
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