Anti-HSF2 Antibody [3E2] (A305122)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF2 Antibody [3E2] (A305122), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rat (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of human K562 cell lysates showing detection of HSF2 protein using Anti-HSF2 Antibody [3E2] (A305122) at 1:1,000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF2 Antibody [3E2] (A305122), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.