Western blot analysis of Cyanobacteria Cell lysates showing detection of HSP16.6 protein using Anti-HSP16.6 Antibody (A304829) at 1:5,000 for 1 hour at room temperature. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit at 1:10000. Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose.
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