This antibody does not cross-react with HSP27 or alpha Crystallin.
Immunogen
Human HSP22.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~22 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha-crystallin C chain, CRYAC, E2-induced gene 1 protein, E2IG1, Heat shock protein beta-8, Heat shock protein family B member 8, Protein kinase H11, Small stress protein-like protein HSP22
Immunohistochemistry analysis of mouse spinal cord. The Primary Antibody used was Anti-HSP22 Antibody (A304777) at 1:100. The secondary antibody used was Alexa Fluor 488 Goat Anti-Rabbit. DAPI merged with Alexa 488.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSP22 Antibody (A304777) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis positive, dermal staining.
Western blot analysis of rat Skeletal muscle lysates showing detection of HSP22 protein using Anti-HSP22 Antibody (A304777) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-HSP22 Antibody (A304777), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Nuclear Staining.
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