Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134)

Name: Anti-HSP90 alpha + beta Antibody [Hyb-K41220A]
Brand: Antibodies.com
SKU: A305134
Price: 405.0 GBP
Availability: InStock

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$540

Mouse monoclonal [Hyb-K41220A] antibody to HSP90 alpha + beta for WB, IHC, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-HSP90 alpha + beta Antibody [Hyb-K41220A]

Description

Mouse monoclonal [Hyb-K41220A] antibody to HSP90 alpha + beta.

Specificity

This antibody detects both alpha (inducible) and beta (constitutively-expressed) forms.

Applications

WB, IHC, ICC/IF, ELISA

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Yeast

Immunogen

Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304.

Host

Mouse

Clonality

Monoclonal

Clone ID

Hyb-K41220A

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Heat shock 84 kDa, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, HSP 84, HSP 86, HSP 90, HSP84, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCA, HSPCB, LAP-2, Lipopolysaccharide-associated protein 2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunofluorescence - Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

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Western Blot - Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134)

Western blot analysis of human Cell lysates showing detection of Hsp90 alpha protein using Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.

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Immunofluorescence - Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 alpha + beta Antibody [Hyb-K41220A] (A305134), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Melanosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

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