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Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617)

Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) - Antibodies.com Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1169] antibody to Hsp90 alpha + Hsp90 beta for WB and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169]
Description
Rabbit monoclonal [ARC1169] antibody to Hsp90 alpha + Hsp90 beta.
Applications
WB, IP
Dilutions
WB: 1:500-1:2,000, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 533-732 of human Hsp90a/ß (P07900).
Sequence
LKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1169
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) - Antibodies.comEnlarge Image

Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617)

Western blot analysis of extracts of various cell lines, using Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) - Antibodies.comEnlarge Image

Western Blot - Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617). This Western blot was performed on the immunoprecipitate using Anti-Hsp90 alpha + Hsp90 beta Antibody [ARC1169] (A306617) at a dilution of 1:1000.
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