Detection of human IgG1 in monkey serum, using Anti-Human IgG1 Antibody [RM117] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
ELISA showing Anti-Human Ig Light Chain Antibody [RM129] reacts only to kappa and lambda light chain of all human immunoglobulins, and not to mouse IgG, rat IgG, or goat IgG.
ELISA showing Anti-Human Ig Light Chain Antibody [RM129] reacts only to kappa and lambda light chain of all human immunoglobulins, and not to mouse IgG, rat IgG, or goat IgG.
ELISA showing Anti-Human Ig Light Chain Antibody [RM129] does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Anti-Human Ig Light Chain Antibody [RM129] and a monkey IgG binding antibody (positive control) was used as the detection antibody.
A titer Sandwich ELISA using Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody. The plate, coated with Anti-gamma Heavy Chain Antibody as the capture antibody, was loaded with different amounts of human IgG. A serial dilution of Anti-Human Ig Light Chain Antibody [RM129] (Biotin) was used as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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