This antibody recognises an epitope contained in the CH2 domain of the Fc fragment of Human IgA.
Applications
ELISA
Dilutions
ELISA: 1:500 - 1:1,000
Reactivity
Human
Immunogen
Purified human IgA.
Host
Mouse
Clonality
Monoclonal
Clone ID
15D6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Sandwich ELISA analysis of human IgA binding using Anti-Human IgA Heavy Chain Antibody [Mc24-2E11] (A281069) as a capture reagent and biotinylated Anti-Human IgA Antibody [15D6] (A279032) as a detection reagent with Native Human Secretory IgA Protein (A320429) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions.
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