Sandwich ELISA using Anti-Human IgD Antibody [RM123] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows Anti-Human IgD Antibody [RM123] reacts to IgD from human plasma and IgD? from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgD Antibody [RM123] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using Anti-Human IgD Antibody [RM123]. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of Anti-Human IgD Antibody [RM123] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Proteins predicted to interact with IgD
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.