Sandwich ELISA using Anti-Human IgE Antibody [RM122] as the capture antibody (25ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows Anti-Human IgE Antibody [RM122] reacts only to human IgE? from human myeloma plasma and the IgE from human plasma. There is no cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml or 10ng/ml of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using Anti-Human IgE Antibody [RM122]. The plate was coated with different amounts of human IgE. A serial dilution of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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