Mouse monoclonal [EM-07] antibody to Human IgG Fc (FITC).
Specificity
This antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-4 µg/ml
Reactivity
Human
Immunogen
Fusion protein of human IgG Fc fragment.
Host
Mouse
Clonality
Monoclonal
Clone ID
EM-07
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow Cytometry - Anti-Human IgG Fc Antibody [EM-07] (FITC) (A86764)
Flow cytometry surface staining pattern of human peripheral whole blood labeling Human IgG Fc with Anti-Human IgG Fc Antibody [EM-07] (FITC) (A86764), (3 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-Human IgG Fc Antibody [EM-07] (FITC) (A86764)
Separation of human IgG (Fc) positive CD19 negative lymphocytes (red-filled) from CD19 positive IgG (Fc) negative lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling Human IgG Fc with Anti-Human IgG Fc Antibody [EM-07] (FITC) (A86764), (3 µl reagent / 100 µl of peripheral whole blood).