ELISA of human immunoglobulins shows Anti-Human Lambda Light Chain Antibody [RM127] reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immuno-globulins. 200ng/ml or 50ng/ml of Anti-Human Lambda Light Chain Antibody [RM127] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using Anti-Human Lambda Light Chain Antibody [RM127]. The plate was coated with different amounts of human IgG3?. A serial dilution of Anti-Human Lambda Light Chain Antibody [RM127] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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