Anti-Human Serum Albumin Antibody [AL-01] (Biotin)
Description
Mouse monoclonal [AL-01] antibody to Human Serum Albumin (Biotin).
Specificity
This antibody only recognizes human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; produced in the liver. No cross-reactivity with other serum proteins was observed.
Applications
IHC-P, ELISA, RIA, WB
Dilutions
WB: Positive Control: human plasma, recommended antibody dilution: 2-5 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Rabbit, Mouse, Pig or Cow.
Immunogen
Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.
Host
Mouse
Clonality
Monoclonal
Clone ID
AL-01
Isotype
IgG1
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
65-67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Anti-Human Serum Albumin Antibody [AL-01] (Biotin) (A86269)
Western blotting analysis of human serum albumin using Anti-Human Serum Albumin Antibody [AL-01] (Biotin) (A86269) on human plasma and lysate of HeLa cell line (albumin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of biotinylated mouse anti-human albumin monoclonal antibody followed by IRDye800-conjugated streptavidin. Albumin was detected at approximately 50-67 kDa.
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