Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody [ARC2461] (A308743)

Western blot analysis of extracts of various cell lines, using Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody [ARC2461] (A308743) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of Mouse stomach, using Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody [ARC2461] (A308743) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody [ARC2461] (A308743) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody [ARC2461] (A308743) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.