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Anti-I Afadin Antibody (A308730)

Western Blot - Anti-I Afadin Antibody (A308730) - Antibodies.com Immunohistochemistry - Anti-I Afadin Antibody (A308730) - Antibodies.com
This product is discontinued

Anti-I Afadin Antibody (A308730) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-I Afadin Antibody
Description
Rabbit polyclonal antibody to I Afadin.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1510-1680 of human MLLT4 (NP_001353249.1).
Sequence
SLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
200 - 250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-I Afadin Antibody (A308730) - Antibodies.comEnlarge Image

Western Blot - Anti-I Afadin Antibody (A308730)

Western blot analysis of extracts of various cell lines, using Anti-I Afadin Antibody (A308730) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-I Afadin Antibody (A308730) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-I Afadin Antibody (A308730)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-I Afadin Antibody (A308730) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.