This antibody recognizes an extracellular epitope in the D2 domain of CD50, a 120-130 kDa type I membrane protein of the immunoglobulin supergene family, expressed on leukocytes, endothelial cells, and Langerhans cells. It is negative on platelets and erythrocytes.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 4 µg/ml.
Reactivity
Human
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-171
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
120-130 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human lymphocytes (red-filled) from CD50 negative blood debris (black-dashed) in flow cytometry surface staining of peripheral whole blood, labeling ICAM3 with Anti-ICAM3 Antibody [MEM-171] (A85863), (concentration in sample 0,6 µg/ml, GAM APC).
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