Anti-IDH3B Antibody (A89844)

Name: Anti-IDH3B Antibody
Brand: Antibodies.com
SKU: A89844
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to IDH3B for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-IDH3B Antibody

Description

Rabbit polyclonal antibody to IDH3B.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2).

Sequence

ASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-IDH3B Antibody (A89844)

Western blot analysis of extracts of Rat heart, using Anti-IDH3B Antibody (A89844) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-IDH3B Antibody (A89844)

Western blot analysis of extracts of various cell lines, using Anti-IDH3B Antibody (A89844) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-IDH3B Antibody (A89844)

Immunofluorescence analysis of L929 cells using Anti-IDH3B Antibody (A89844) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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